Simultaneous Detection of Distinct Ubiquitin Chain Topologies by 19F NMR
نویسندگان
چکیده
منابع مشابه
Simultaneous Detection of Distinct Ubiquitin Chain Topologies by 19F NMR
The dynamic interplay between ubiquitin (Ub) chain construction and destruction is critical for the regulation of many cellular pathways. To understand these processes, it would be ideal to simultaneously detect different Ub chains as they are created and destroyed in the cell. This objective cannot be achieved with existing detection strategies. Here, we report on the use of 19F Nuclear Magnet...
متن کاملLead increases free Ca2+ concentration in cultured osteoblastic bone cells: simultaneous detection of intracellular free Pb2+ by 19F NMR.
Lead (Pb) has been shown to perturb Ca-mediated cellular processes. However, to date, a direct effect of Pb on intracellular free Ca2+ concentration ([Ca2+]i) has not been demonstrated. 19F NMR in combination with 1,2-bis(2-amino-5-fluorophenoxy)ethane-N,N,N',N'-tetraacetic acid (5F-BAPTA) was used to simultaneously measure [Ca2+]i and intracellular free Pb2+ concentration ([Pb2+]i) in the rat ...
متن کاملextremal region detection guided by maxima of gradient magnitude
a problem of computer vision applications is to detect regions of interest under dif- ferent imaging conditions. the state-of-the-art maximally stable extremal regions (mser) detects affine covariant regions by applying all possible thresholds on the input image, and through three main steps including: 1) making a component tree of extremal regions’ evolution (enumeration), 2) obtaining region ...
1317 Detection of dexamethasone in the aqueous humour by 1H and 19F NMR spectroscopy
Background and purpose: A likely explanation for the increase in lens fluorescence with aging and the acceleration of this process in patients with diabetes, is that lens proteins are altered by non-enzymatic glycosylation and the further steps of the Maillard reaction. Thus, the lens fluorescence intensity appears to represent a cumulative index of glycemia over the entire lifetime of the subj...
متن کاملMonitoring the Disassembly of Virus-like Particles by 19F-NMR
Virus-like particles (VLPs) are stable protein cages derived from virus coats. They have been used extensively as biomolecular platforms, e.g., nanocarriers or vaccines, but a convenient in situ technique is lacking for tracking functional status. Here, we present a simple way to monitor disassembly of 19F-labeled VLPs derived from bacteriophage Qβ by 19F NMR. Analysis of resonances, under a ra...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: ACS Chemical Biology
سال: 2014
ISSN: 1554-8929,1554-8937
DOI: 10.1021/cb500589c